Pyrrolidiini

Kemiallinen kuvaus

Chemical : c ₁₂ h ₂₁ n ₃ > O ₃

system named : n6 - {[(2R, 3R) -3-methyl-3,4-dihydro-2h-pyrrol -2-yl] carbonyl} -l-lysine

N6-[(2R,3R)-3-metyyli-3,4-dihydro-2-hydropyrroli-2-formyyli]-L-amiinihappo

: PYL O

: PYL O

Löytöprosessi

8 researchers from two research teams from Ohio State University identify the world's 22nd natural amino acid-pyrolily alleysine (Pyrrolysine) encoded by genetic genes. Since 1995, the Krzycki Study Group has found an unexpected phenomenon in the study of methyl methane (MMA, DMA, TMA) methyltransferase gene: there is a box of ingredients in the gene. Take a codon a UAG. Under normal circumstances, the codon should issue a signal to stop the protein synthesis, but there is no synthesis of the protein in the gene. The Krzycki Study Group was studied by mass spectrometry and EDMAN degradation, indicating that the codon encodes a modified lysine. The research team led by Chan and HAO was studied by the MTMB crystal structure of MTMB crystal structure in methane bacteria ( Methanosarcina Barkeri ), found that the amino acid residue encoded by codon UAG The density is different from the amino acid residue encoded by other 21 codon, which is linked (4R, 5R) 4-pyrrolin, a carboxyl group (4R, 5R) 4-pyrrolin. So they said this newly discovered amino acid is L-pyrrolidine.

Biologinen toiminta

Pyrrolidiini löytyy metaani metyyliamiini metyylitransferaasi, sen tiedetään osallistuvan aminohapon proteiinien biologisen synteesin kanssa Standardiaminohappo on erilainen, ja se muodostuu määrittämällä UAG-kodoni. Sitä vastoin spesifinen pyrrolidyyli-tRNA-syntetaasi (PYLRS) ja pyrrolidiini-TRNA (TRNA (superscript PYL)) ovat myös erilaisia ​​kuin klassinen TRNA. Metaanibasillien muodostuminen tuottaa pyrrolidyyli-tRNA:ta (PYL-TRNA (PYL)) suoraa reittiä ja epäsuoraa reittiä pitkin (PYL-TRNA (superscript PYL)), joka voi myös kulkea mRNA:n ja muiden säännöllisten erityisrakenteen läpi. UAG-koodaus kodonin tai pyrrolidiinin lopettamiseksi.