Základní pojmy
Geneexpressiondatacontainsinformationaboutgeneactivity,whichcanreflectthecurrentphysiologicalstateofcells,suchaswhethercellsareinanormalordeterioratingstate,whetherdrugsareeffectiveontumorcells,etc..Theanalysisofgeneexpressiondatacanobtaingenefunctionandgeneexpressionregulationinformation.ThisisoneofthemajorchallengesofbioinformaticsandoneofthekeyreasonswhyDNAmicroarrayscanbewidelyusedinthefieldofbiomedicine.
Funkce a směry výzkumu
Funkce
Theobjectofgeneexpressiondataanalysisisadatamatrixcomposedofallorpartofthegeneexpressiondataunderdifferentconditions.Throughtheanalysisofthisdatamatrix,somebiologicalquestionscanbeanswered.Theanswerstothesequestions,combinedwithotherbiologicalknowledgeanddata,willhelpclarifygeneexpressionregulationpathwaysandregulatorynetworks.Revealinggeneregulationpathwaysandnetworksisthecommongoalofbiologyandbioinformatics,andisthecorecontentofsystemsbiologyresearch.
Směr výzkumu
1.Analyzujte úroveň exprese jednotlivého genu
Accordingtothechangeofgeneexpressionlevelunderdifferentexperimentalconditions,Tojudgeitsfunction,forexample,thespecificgenesrelatedtotumortypingcanbedeterminedbasedonthesignificanceoftheexpressiondifference.Theanalysismethodsusedincludehypothesistestinginstatisticsandsoon.
2.Uvažujte o kombinacích
Groupgenestostudytheircommonfunctions,interactions,andcoordinatedregulation.Methodssuchasclusteranalysisareoftenused.
3.Zkuste odvodit potenciální genovou regulační síť
Interprettheobservedgeneexpressiondatafromthemechanism.Reverseengineeringmethodsareoftenused.